Immobilization of Candida antarctica Lipase B on Silicone Nanofilaments

Candida antarctica lipase B was immobilized on a series of silicone nanofilament-coated matrices different porosities. In addition to creating more open surface, SNF’s hydrophobicity allows for simple immobilization pathway via adsorption. order study the impact nanostructure, performance compared with control samples lacking SNFs. For all materials, surface characterized BET measurements, and enzyme measured as well catalytic activity. Enzyme loads ranged between 3.85% w / 2.53% id="M2"> decreased decreasing area carrier material from 200 m2/g 0.04 m2/g, while activity per increases 824 U 2040 U. The data suggest that coating seals off inner surfaces, forcing be at accessible positions allowing higher enzyme. Optimization conditions allowed us create thinner layer which further improved 3129 While this is comparable commercial Novozyme 435 3073 U, SNF-based system performs catalysis in thin around 13 μm. A favorite application is, example, creation enzyme-based detection systems, where high up 89622 id="M3"> · mg/m2 would lead signal strength.